So, the sliminess on amphibians is more than just goop; it’s basically how these animals survive. The slime is also a great environment for bacteria and microscopic fungi to live. This community of microorganisms is called a microbiome. These microbiomes are extremely beneficial, and can defend an animal against disease, infection and more. However, anything that messes with this slime, messes with the ability of an amphibian to breathe.
In fact, a deadly fungus called chytridiomycosis, commonly known as amphibian chytrid, is killing thousands of amphibians across the globe by doing just that. A decline of amphibian species in an environment causes a lot of issues. Beyond serving as an important food source for larger animals, amphibians act as nature’s pest control by eating all sorts of insects (including ones that are harmful to humans, like mosquitoes). In some ecosystems, the amphibian population is greater than all other vertebrate animal species present. The health of amphibians can alter an entire ecosystem very quickly.
Because my colleagues and I work at a zoo with a large amphibian collection, we have access to a unique collection of slime—hundreds of amphibian species representing 250 million years of evolution are at our disposal. These amphibians live at the Reptile Discovery Center and Amazonia. If we could understand more about amphibians’ microbiomes, it might be the key to saving species from threats like chytrid.
In conservation genomics, we study the building blocks of life, like DNA. As you probably know from TV crime shows like "CSI," scientists can extract DNA from a variety of organic materials, including bones, blood, hair, and skin. We can do that with conservation, too—only instead of using DNA at crime scenes to identify culprits and victims, we analyze the DNA of animal species to help save them. By studying the DNA of the tiny, microscopic creatures that live in amphibian slime, we can find out more about how they help amphibians survive, live, and breathe. So, if we want to save amphibians, we’re going to need slime... and lots of it.
Collecting slime is just what it sounds like, with a few extra steps to ensure the amphibians—frogs, in this case—aren’t stressed. First, we put on our gloves and pick up the frogs. Next, we take a sterile cotton swab and gently roll it on the frog's skin to collect a small sample of slime. Then, we place the frog in a small bag of water and wait for the slime to slough off. And once that's done, we can use the samples of this “bath” water to create experiments to measure the slime’s ability to ward off the chytrid fungus.